S-Layer Nanosheet Binding of Zn and Gd

Publicly accessible License 

This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.

A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),

References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.

Citation Formats

Lawrence Berkeley National Laboratory. (2016). S-Layer Nanosheet Binding of Zn and Gd [data set]. Retrieved from https://dx.doi.org/10.15121/1372392.
Export Citation to RIS
Ajo-Franklin, Caroline, Charrier, Marimikel, and Yang, Li. S-Layer Nanosheet Binding of Zn and Gd. United States: N.p., 15 Apr, 2016. Web. doi: 10.15121/1372392.
Ajo-Franklin, Caroline, Charrier, Marimikel, & Yang, Li. S-Layer Nanosheet Binding of Zn and Gd. United States. https://dx.doi.org/10.15121/1372392
Ajo-Franklin, Caroline, Charrier, Marimikel, and Yang, Li. 2016. "S-Layer Nanosheet Binding of Zn and Gd". United States. https://dx.doi.org/10.15121/1372392. https://gdr.openei.org/submissions/951.
@div{oedi_951, title = {S-Layer Nanosheet Binding of Zn and Gd}, author = {Ajo-Franklin, Caroline, Charrier, Marimikel, and Yang, Li.}, abstractNote = {This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.

A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),

References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
}, doi = {10.15121/1372392}, url = {https://gdr.openei.org/submissions/951}, journal = {}, number = , volume = , place = {United States}, year = {2016}, month = {04}}
https://dx.doi.org/10.15121/1372392

Details

Data from Apr 15, 2016

Last updated Jan 9, 2020

Submitted Jul 20, 2017

Organization

Lawrence Berkeley National Laboratory

Contact

Caroline Ajo-Franklin

510.486.4299

Authors

Caroline Ajo-Franklin

Lawrence Berkeley National Laboratory

Marimikel Charrier

Lawrence Berkeley National Laboratory

Li Yang

Lawrence Berkeley National Laboratory

DOE Project Details

Project Name Engineering Thermophilic Microorganisms to Selectively Extract Strategic Metals from Low Temperature Geothermal Brines

Project Lead Holly Thomas

Project Number FY15 AOP 2514

Share

Submission Downloads