S-Layer Nanosheet Binding of Zn and Gd

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This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.

A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),

References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.

Citation Formats

TY - DATA AB - This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods. A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000), References: Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753. Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886. AU - Ajo-Franklin, Caroline A2 - Charrier, Marimikel A3 - Yang, Li DB - Geothermal Data Repository DP - Open EI | National Renewable Energy Laboratory DO - 10.15121/1372392 KW - geothermal KW - energy KW - mineral recovery KW - synthetic biology KW - S-layer KW - metal-binding domain KW - nanosheet KW - brine study KW - binding KW - chemistry KW - Gadolinium KW - zinc KW - gd3 KW - zn2 KW - ion LA - English DA - 2016/04/15 PY - 2016 PB - Lawrence Berkeley National Laboratory T1 - S-Layer Nanosheet Binding of Zn and Gd UR - https://doi.org/10.15121/1372392 ER -
Export Citation to RIS
Ajo-Franklin, Caroline, et al. S-Layer Nanosheet Binding of Zn and Gd. Lawrence Berkeley National Laboratory, 15 April, 2016, Geothermal Data Repository. https://doi.org/10.15121/1372392.
Ajo-Franklin, C., Charrier, M., & Yang, L. (2016). S-Layer Nanosheet Binding of Zn and Gd. [Data set]. Geothermal Data Repository. Lawrence Berkeley National Laboratory. https://doi.org/10.15121/1372392
Ajo-Franklin, Caroline, Marimikel Charrier, and Li Yang. S-Layer Nanosheet Binding of Zn and Gd. Lawrence Berkeley National Laboratory, April, 15, 2016. Distributed by Geothermal Data Repository. https://doi.org/10.15121/1372392
@misc{GDR_Dataset_951, title = {S-Layer Nanosheet Binding of Zn and Gd}, author = {Ajo-Franklin, Caroline and Charrier, Marimikel and Yang, Li}, abstractNote = {This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.

A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),

References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
}, url = {https://gdr.openei.org/submissions/951}, year = {2016}, howpublished = {Geothermal Data Repository, Lawrence Berkeley National Laboratory, https://doi.org/10.15121/1372392}, note = {Accessed: 2025-04-23}, doi = {10.15121/1372392} }
https://dx.doi.org/10.15121/1372392

Details

Data from Apr 15, 2016

Last updated Jan 9, 2020

Submitted Jul 20, 2017

Organization

Lawrence Berkeley National Laboratory

Contact

Caroline Ajo-Franklin

510.486.4299

Authors

Caroline Ajo-Franklin

Lawrence Berkeley National Laboratory

Marimikel Charrier

Lawrence Berkeley National Laboratory

Li Yang

Lawrence Berkeley National Laboratory

DOE Project Details

Project Name Engineering Thermophilic Microorganisms to Selectively Extract Strategic Metals from Low Temperature Geothermal Brines

Project Lead Holly Thomas

Project Number FY15 AOP 2514

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