S-Layer Nanosheet Binding of Zn and Gd
This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.
A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),
References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
Citation Formats
Lawrence Berkeley National Laboratory. (2016). S-Layer Nanosheet Binding of Zn and Gd [data set]. Retrieved from https://dx.doi.org/10.15121/1372392.
Ajo-Franklin, Caroline, Charrier, Marimikel, and Yang, Li. S-Layer Nanosheet Binding of Zn and Gd. United States: N.p., 15 Apr, 2016. Web. doi: 10.15121/1372392.
Ajo-Franklin, Caroline, Charrier, Marimikel, & Yang, Li. S-Layer Nanosheet Binding of Zn and Gd. United States. https://dx.doi.org/10.15121/1372392
Ajo-Franklin, Caroline, Charrier, Marimikel, and Yang, Li. 2016. "S-Layer Nanosheet Binding of Zn and Gd". United States. https://dx.doi.org/10.15121/1372392. https://gdr.openei.org/submissions/951.
@div{oedi_951, title = {S-Layer Nanosheet Binding of Zn and Gd}, author = {Ajo-Franklin, Caroline, Charrier, Marimikel, and Yang, Li.}, abstractNote = {This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.
A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),
References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
}, doi = {10.15121/1372392}, url = {https://gdr.openei.org/submissions/951}, journal = {}, number = , volume = , place = {United States}, year = {2016}, month = {04}}
https://dx.doi.org/10.15121/1372392
Details
Data from Apr 15, 2016
Last updated Jan 9, 2020
Submitted Jul 20, 2017
Organization
Lawrence Berkeley National Laboratory
Contact
Caroline Ajo-Franklin
510.486.4299
Authors
Keywords
geothermal, energy, mineral recovery, synthetic biology, S-layer, metal-binding domain, nanosheet, brine study, binding, chemistry, Gadolinium, zinc, gd3, zn2, ionDOE Project Details
Project Name Engineering Thermophilic Microorganisms to Selectively Extract Strategic Metals from Low Temperature Geothermal Brines
Project Lead Holly Thomas
Project Number FY15 AOP 2514