S-Layer Nanosheet Binding of Zn and Gd
This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.
A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),
References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
Citation Formats
TY - DATA
AB - This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.
A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),
References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
AU - Ajo-Franklin, Caroline
A2 - Charrier, Marimikel
A3 - Yang, Li
DB - Geothermal Data Repository
DP - Open EI | National Renewable Energy Laboratory
DO - 10.15121/1372392
KW - geothermal
KW - energy
KW - mineral recovery
KW - synthetic biology
KW - S-layer
KW - metal-binding domain
KW - nanosheet
KW - brine study
KW - binding
KW - chemistry
KW - Gadolinium
KW - zinc
KW - gd3
KW - zn2
KW - ion
LA - English
DA - 2016/04/15
PY - 2016
PB - Lawrence Berkeley National Laboratory
T1 - S-Layer Nanosheet Binding of Zn and Gd
UR - https://doi.org/10.15121/1372392
ER -
Ajo-Franklin, Caroline, et al. S-Layer Nanosheet Binding of Zn and Gd. Lawrence Berkeley National Laboratory, 15 April, 2016, Geothermal Data Repository. https://doi.org/10.15121/1372392.
Ajo-Franklin, C., Charrier, M., & Yang, L. (2016). S-Layer Nanosheet Binding of Zn and Gd. [Data set]. Geothermal Data Repository. Lawrence Berkeley National Laboratory. https://doi.org/10.15121/1372392
Ajo-Franklin, Caroline, Marimikel Charrier, and Li Yang. S-Layer Nanosheet Binding of Zn and Gd. Lawrence Berkeley National Laboratory, April, 15, 2016. Distributed by Geothermal Data Repository. https://doi.org/10.15121/1372392
@misc{GDR_Dataset_951,
title = {S-Layer Nanosheet Binding of Zn and Gd},
author = {Ajo-Franklin, Caroline and Charrier, Marimikel and Yang, Li},
abstractNote = {This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.
A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),
References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
},
url = {https://gdr.openei.org/submissions/951},
year = {2016},
howpublished = {Geothermal Data Repository, Lawrence Berkeley National Laboratory, https://doi.org/10.15121/1372392},
note = {Accessed: 2025-07-09},
doi = {10.15121/1372392}
}
https://dx.doi.org/10.15121/1372392
Details
Data from Apr 15, 2016
Last updated Jan 9, 2020
Submitted Jul 20, 2017
Organization
Lawrence Berkeley National Laboratory
Contact
Caroline Ajo-Franklin
510.486.4299
Authors
Keywords
geothermal, energy, mineral recovery, synthetic biology, S-layer, metal-binding domain, nanosheet, brine study, binding, chemistry, Gadolinium, zinc, gd3, zn2, ionDOE Project Details
Project Name Engineering Thermophilic Microorganisms to Selectively Extract Strategic Metals from Low Temperature Geothermal Brines
Project Lead Holly Thomas
Project Number FY15 AOP 2514
