Rare Earth Desorption Test with Citrate and Bicarbonate
LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.
Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.
Citation Formats
Lawrence Livermore National Laboratory. (2017). Rare Earth Desorption Test with Citrate and Bicarbonate [data set]. Retrieved from https://dx.doi.org/10.15121/1452718.
Jiao, Yongqin, Brewer, Aaron, and Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate. United States: N.p., 01 Jun, 2017. Web. doi: 10.15121/1452718.
Jiao, Yongqin, Brewer, Aaron, & Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate. United States. https://dx.doi.org/10.15121/1452718
Jiao, Yongqin, Brewer, Aaron, and Park, Dan. 2017. "Rare Earth Desorption Test with Citrate and Bicarbonate". United States. https://dx.doi.org/10.15121/1452718. https://gdr.openei.org/submissions/965.
@div{oedi_965, title = {Rare Earth Desorption Test with Citrate and Bicarbonate}, author = {Jiao, Yongqin, Brewer, Aaron, and Park, Dan.}, abstractNote = {LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.
Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.}, doi = {10.15121/1452718}, url = {https://gdr.openei.org/submissions/965}, journal = {}, number = , volume = , place = {United States}, year = {2017}, month = {06}}
https://dx.doi.org/10.15121/1452718
Details
Data from Jun 1, 2017
Last updated Jun 14, 2018
Submitted Sep 5, 2017
Organization
Lawrence Livermore National Laboratory
Contact
Yongqin Jiao
925.422.4482
Authors
Keywords
geothermal, energy, adsorption, desorption, rare earth, citrate, bicarbonate, REE, resource recovery, centrifuge, brine, lbt, lanthanide binding tagDOE Project Details
Project Name Extraction of Rare Earth Metals from Geothermal Fluids using Bioengineered Microbes
Project Lead Holly Thomas
Project Number LLNL FY17 AOP 2.5.1.12
