Rare Earth Desorption Test with Citrate and Bicarbonate

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LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.

Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.

Citation Formats

Lawrence Livermore National Laboratory. (2017). Rare Earth Desorption Test with Citrate and Bicarbonate [data set]. Retrieved from https://dx.doi.org/10.15121/1452718.
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Jiao, Yongqin, Brewer, Aaron, and Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate. United States: N.p., 01 Jun, 2017. Web. doi: 10.15121/1452718.
Jiao, Yongqin, Brewer, Aaron, & Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate. United States. https://dx.doi.org/10.15121/1452718
Jiao, Yongqin, Brewer, Aaron, and Park, Dan. 2017. "Rare Earth Desorption Test with Citrate and Bicarbonate". United States. https://dx.doi.org/10.15121/1452718. https://gdr.openei.org/submissions/965.
@div{oedi_965, title = {Rare Earth Desorption Test with Citrate and Bicarbonate}, author = {Jiao, Yongqin, Brewer, Aaron, and Park, Dan.}, abstractNote = {LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.

Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.}, doi = {10.15121/1452718}, url = {https://gdr.openei.org/submissions/965}, journal = {}, number = , volume = , place = {United States}, year = {2017}, month = {06}}
https://dx.doi.org/10.15121/1452718

Details

Data from Jun 1, 2017

Last updated Jun 14, 2018

Submitted Sep 5, 2017

Organization

Lawrence Livermore National Laboratory

Contact

Yongqin Jiao

925.422.4482

Authors

Yongqin Jiao

Lawrence Livermore National Laboratory

Aaron Brewer

Lawrence Livermore National Laboratory

Dan Park

Lawrence Livermore National Laboratory

DOE Project Details

Project Name Extraction of Rare Earth Metals from Geothermal Fluids using Bioengineered Microbes

Project Lead Holly Thomas

Project Number LLNL FY17 AOP 2.5.1.12

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