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Rare Earth Desorption Test with Citrate and Bicarbonate

Jun 01, 2017
65.97 kB
Publicly accessible
LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 u...
Authors
Jiao, Y. et al Lawrence Livermore National Laboratory
geothermalenergyadsorptiondesorptionrare earthcitratebicarbonatereeresource recoverycentrifugebrinelbtlanthanide binding tag

Rare Earth Element Biomining from the Great Salt Lake Brine Using Engineered E. Coli

Jun 04, 2017
207.33 kB
Publicly accessible
This data describes rare earth element adsorption onto E. coli cells engineered to express a lanthanide binding tag (LBT). We used a Great Salt Lake synthetic solution as the background matrix with Tb added to 1-10,000 ppb, concentrations much lower than the competing ions presen...
Authors
Jiao, Y. et al Lawrence Livermore National Laboratory
geothermalenergyrare earth elementadsorptiongeofluidreebrinebiomininge. coligreat salt lakebrine study

Rare Earth Adsorption and Desorption with PEGDA Beads

Mar 01, 2017
61.2 kB
Publicly accessible
We synthesized PEGDA polymer hydrogel beads for cell embedding and compared REE biosorption with these beads via a gravity-driven flow through setup. One way to set up a flow through system is by cell encapsulation into polymer beads with a column setup similar to that used in th...
Authors
Jiao, Y. et al Lawrence Livermore National Laboratory
geothermalenergyhydrogelpegdarare earthbiosorptiondesorptionadsorptioncolumngreat salt lakebrinelanthanide binding taglbt cellspeg diacrylate