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Rare Earth Desorption Test with Citrate and Bicarbonate

Jun 01, 2017
65.97 kB
Publicly accessible
LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 u...
Authors
Jiao, Y. et al Lawrence Livermore National Laboratory
geothermalenergyadsorptiondesorptionrare earthcitratebicarbonatereeresource recoverycentrifugebrinelbtlanthanide binding tag

Rare Earth Element Biomining from the Great Salt Lake Brine Using Engineered E. Coli

Jun 04, 2017
207.33 kB
Publicly accessible
This data describes rare earth element adsorption onto E. coli cells engineered to express a lanthanide binding tag (LBT). We used a Great Salt Lake synthetic solution as the background matrix with Tb added to 1-10,000 ppb, concentrations much lower than the competing ions presen...
Authors
Jiao, Y. et al Lawrence Livermore National Laboratory
geothermalenergyrare earth elementadsorptiongeofluidreebrinebiomininge. coligreat salt lakebrine study

Rare Earth Adsorption and Desorption with PEGDA Beads

Mar 01, 2017
61.2 kB
Publicly accessible
We synthesized PEGDA polymer hydrogel beads for cell embedding and compared REE biosorption with these beads via a gravity-driven flow through setup. One way to set up a flow through system is by cell encapsulation into polymer beads with a column setup similar to that used in th...
Authors
Jiao, Y. et al Lawrence Livermore National Laboratory
geothermalenergyhydrogelpegdarare earthbiosorptiondesorptionadsorptioncolumngreat salt lakebrinelanthanide binding taglbt cellspeg diacrylate

Recovery of Rare Earth Elements from Geothermal Fluids through Bacterial Cell Surface Adsorption

Jan 01, 2018
7.18 MB
Publicly accessible
We summarized the FY17 and part of FY18 results of the analysis of the effect of several parameters (e.g., total dissolved solids, specific competing metals, pH, and temperature) on REE recovery from geothermal brine in a manuscript that was submitted to Environmental Science & Te...
Authors
Jiao, Y. et al Lawrence Livermore National Laboratory
geothermalenergyreebrinefluidrare earthelementsbacteriacellsurfaceadsorptionbioengineeringmicrobemicrobialbiosorptionbioadsorptionlanthanide bindingtaglbtgeofluide. coligeochemicalgeochemistryfactors

DASH Slow Strain Rates from Brady Hot Springs Geothermal Field during PoroTomo Deployment Period

Jun 27, 2018
4.98 GB
Publicly accessible
This submission contains slow strain rates summed to radians over 30 second intervals [rad/s] derived from horizontal distributed acoustic sensing measurements (DASH) of Brady geothermal field during PoroTomo deployment (2016-Mar-14 to 2016-Mar-26). There is one file correspondin...
Authors
Reinisch, E. et al University of Wisconsin
geothermaldashporotomoslow strainbrady hot springsdashorizontaldistributed acoustic sensingmatlabstrain ratestraincharacterizationgeophysicshydrothermalbradyprocessed datasoftwarerepositorytechnicalreportslow strain rate

Surface Complexation Modeling of Terbium Biosorption onto E. Coli Bacterial Surfaces with Lanthanide Binding Tags

Apr 01, 2018
142.53 kB
Publicly accessible
Lanthanide binding tags (LBTs) have been engineered onto native Escherichia coli (E. coli) bacterial surfaces to enhance extraction and recovery of rare earth elements (REEs). Three strains of E. coli were studied: (1) the native E. coli surface, (2) a mutant E. coli surface with ...
Authors
Jiao, Y. Lawrence Livermore National Laboratory
geothermalenergymodelingterbiumbiosorptione. colibacteriabacterialsurfacelanthanide bindingtaglbtreerare earthbrinefluidgeofluidgeochemicalgeochemistrybioadsorptionbioengineering