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LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 u...
This data describes rare earth element adsorption onto E. coli cells engineered to express a lanthanide binding tag (LBT). We used a Great Salt Lake synthetic solution as the background matrix with Tb added to 1-10,000 ppb, concentrations much lower than the competing ions presen...
We synthesized PEGDA polymer hydrogel beads for cell embedding and compared REE biosorption with these beads via a gravity-driven flow through setup. One way to set up a flow through system is by cell encapsulation into polymer beads with a column setup similar to that used in th...
This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+ or Gd3+-binding domains on these sheets. Their ab...
Study on the use of organic ligands to extract lanthanides from low temperature geothermal water.
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